Our fast, objective, validated method of microbial identification.
Starting with a pure isolate, we perform a DNA extraction, amplify all or a portion of the 16S rRNA gene, and sequence the amplicon using state-of-the-art fluorescent di-deoxy terminator cycle chemistry. The extension products are then separated on an automated DNA sequencer, and the data is compared to our comprehensive and proprietary validated library of known sequences.
Bacterial characterizations and identifications are based on sequencing all or part of the 16S rRNA gene. Yeast and mold identifications are based on sequencing of the D2 expansion segment of the large subunit rRNA gene. Even contaminants that are difficult to isolate, highly unusual, or previously unmatched can now be easily identified in as little as 7 hours upon receipt.
Unlike growth-based methods, every sample can be classified - yielding a
phylogenetic tree that clearly shows the closest genetically-related
organisms. Even if a species-level determination can't be made, the
organism's stable 16S sequence can be used for future isolate comparisons.
In addition, we also supply a wide range of specialized data generation and
analysis services to facilitate tracking and trending of your individual
sites or at a global level.
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