At Accugenix, we help you track and trend your microbial environment with expertise in Strain Typing and Microbial Characterization. Utilizing powerful tools for strain typing is essential when the questions are…

 

What is the source of contamination?

Is this production strain the correct one?

What are the normal floral of our production facility?

 

Completely objective personal service

As a complement to our sequence-based microbial identification services, Accugenix offers strain typing by single or multi locus sequence typing (SLST or MLST) – highly accurate sequence-based strain typing methods. We perform custom DNA sequencing of targets outside of the standard regions sequenced for microbial identification (16S, D2 or ITS2) to provide you with data to resolve differences and/or similarities at the subspecies or strain level. The subspecies or strain level IDs allow you to definitively track the source of contamination or simply to verify your known strains. Not only is there a high level of microbial differentiation available by these methods, the MLST and SLST raw sequence data are electronically retained in compliance with cGMP guidelines and accessible for comparison among your facilities. Contact Technical Support to establish a project-specific procedure and timeline to fit your needs.

Perfect companion tests to rDNA sequence-based identifications

Additionally, Accugenix offers automated ribotyping utilizing the DuPont Qualicon RiboPrinter® for strain-level characterization of bacterial isolates (BacRib). This fragment-based technology utilizes restriction enzymes (typically EcoRI and PvuII) to target and cut regions of the ribosomal RNA genes, resulting in a unique fingerprint of the bacterium. Automated ribotyping is useful for the comparison of restriction fragment patterns within the same species. These patterns can be generated prior to or subsequent to each other and be used to determine the level of similarity between isolates as part of a tracking and trending program.

Applications for Strain Typing

Accugenix recommends these strain typing analyses in situations where it is important to examine the strain relatedness of a set of isolates:

Certify strain identity for the Probiotic industry
Ensure stability of QC organisms
Microbial Mapping
QA/QC- OOS Investigations
Research and Development projects
Root Cause Analysis
Sterility Failures
Track Contamination Events

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